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Open in a separate window Dark grey cell denotes ripening time of the fruits when they were sampled. Plant families and species were checked through the CalFlora website [ 65 ]. Deli cups were covered with fine organdy cloth and fitted with a raised metal grid 2 cm high on the bottom to suppress mold growth. A piece of tissue paper was placed underneath the fruit to absorb any liquid accumulation. Emerged flies were collected every 2β3 d, frozen, and then identified as either D.
Only those flies that emerged within 2 weeks following field collection were counted to exclude the possibility of second-generation flies.
Laboratory Tests 2. Insects All laboratory studies were conducted under controlled conditions, as described above Section 2. A laboratory colony of D. The fly larvae were maintained on a standard cornmeal-based artificial diet using methods described by Dalton et al. Field-collected D. All tests used 1β2-week-old adult female flies that had been housed with males since emergence and therefore assumed to be mated.
All fruits were obtained during fruit sampling described previously; crop fruits were collected from unsprayed fields and carefully examined under microscope to ensure there was no natural infestation by D. Because we pre-determined that D. Additionally, wild plum was punctured using 1. The intact and whole fruit was used as a test unit.
Tests for each fruit species consisted of 22β53 replicates. Replicates were a single fruit or fruit unit exposed to a female D. After the female fly was removed and the number of eggs in the fruit was counted, fruits were kept in cages until the emergence of the flies.